Editor-in-Chief Ufuk Demirkılıç Frequency Quarterly Abbreviation Turk J Vasc Surg Publisher Turkish National Vascular and Endovascular Surgery Society ISSN 2667-4947 E-ISSN 2667-5080

Turkish Journal of Vascular Surgery 2021 , Vol 30 , Issue 2
Vascular endothelium protective property of curcumin
Serdar Bayrak1, Gizem Çalıbaşı-Koçal2, Feriha Toksöz3, Tuğra Gençpınar1, Nazlı Mert Özüpek4, Asuman Feda Bayrak5, Ezgi Daşkın6, Yasemin Başbınar2
1Department of Cardiovascular Surgery, Dokuz Eylül University, Faculty of Medicine, Izmir, Turkey
2Department of Translational Oncology, Dokuz Eylül University, Institute of Oncology, Izmir, Turkey
3Dokuz Eylül Technology Development Zone, Tailor of Science Innovation Biotechnology Inc, Izmir, Turkey
4Department of Basic Oncology, Dokuz Eylül University, Institute of Health Sciences, Izmir, Turkey
5Department of Otolaryngology, Katip Çelebi University, Atatürk Training and Research Hospital, Izmir, Turkey
6Department of Translational Oncology, Dokuz Eylül University, Institute of Health Sciences, Izmir, Turkey
DOI : 10.9739/tjvs.2021.927 Objectives: In the present study, we aimed to examine the efficacy of curcumin on endothelial vascular protection on human umbilical vein endothelial cells (HUVEC) cells in the in vitro setting and to determine effective doses.

Materials and methods: Cytotoxic effect and wound healing activity of curcumin on the HUVEC cell line were evaluated and angiogenesis was studied by tube formation assay. The cytotoxic activity of 0.5 to 8 μM curcumin on HUVEC cell line was tested using the WST-1 cell proliferation assay. In wound healing determination, the scratched cells were incubated with the half maximal effective concentration (EC50) dose of curcumin and wounds were monitored by the JuLI™ Br live cell movie analyzer. Wound gaps were measured using the ImageJ software. To determine the angiogenesis, tube formation assay was performed and the results were analyzed.

Results: The 1.77 μM of curcumin increased the cell viability by 150% after 48 h of treatment. According to the wound healing results, after 48 h of incubation, the control group and 1.77 μM curcumin exhibited 47.1% and 43.8% closure, respectively. The mean maximum and minimum tube lengths were found to be 21,858±3,945 and 12,438±3,817 pixel for curcumin, respectively with low fetal bovine serum (FBS) and curcumin with high FBS, respectively.

Conclusion: Our results show that curcumin is a promising endothelial protective agent for HUVEC cell line. Keywords : Curcumin, cytotoxic effect, endothelium protective, wound healing

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