2Department of Cardiovascular Surgery, Gülhane Training and Research Hospital, Ankara, Turkey
3Department of Physiology, Kırıkkale University Faculty of Medicine, Kırıkkale, Turkey
4Department of Cardiovascular Surgery, Karabük University Faculty of Medicine, Karabük, Turkey DOI : 10.9739/tjvs.2022.1104 Objectives: This study aims to examine the impact of curcumin on erythrocyte deformability in a mouse model that can be used for short-term ischemia/reperfusion (IR) injury.
Materials and methods: A total of 30 mice were divided into five groups of six animals in each. The mice were subjected to unilateral hind limb ischemia on the femoral vessels for 2 h. Reperfusion was achieved for the next 2 h. Curcumin (100 mg·kg-1) was administered intraperitoneally (CUR and IR/CUR groups). The mice were euthanized by intraperitoneal administration of ketamine (100 mg·kg-1) and blood was drawn from the heart. A constant flow filter system was used to assess erythrocyte deformability.
Results: There was a statistically significant difference in the mean deformability index among the groups. The IR group (2.1±0.3) significantly higher than the mean of control group (1.6±0.2), CUR group (1.6±0.1) and DMSO group (1.6±0.2). The deformability index values of the IR/CUR group approach the control group through the application of the curcumin (1.8±0.2).
Conclusion: Our study showed negative effects of IR on erythrocyte deformability in mice. Also, curcumin had positive effects by reversing the undesirable effects of IR.
Keywords : Curcumin, erythrocyte deformability, ischemia-reperfusion