Editor-in-Chief Ufuk Demirkılıç Frequency Quarterly Abbreviation Turk J Vasc Surg Publisher Turkish National Vascular and Endovascular Surgery Society E-ISSN 2667-5080

Turkish Journal of Vascular Surgery 2022 , Vol 31 , Issue 1
The role of fetuin-A in vascular aging in the rat aorta
Özgür Barış1, Gülçin Gacar2, Seda Zehra Ünal Halbutoğulları3, Orhan Fındık4, Zeynep Ece Utkan Korun2, Yusufhan Yazır5, Tijen Utkan6, Feride İpek Komşuoğlu Çelikyurt6, Emre Demir Benli7
1Department of Cardiovascular Surgery, Kocaeli University, Faculty of Medicine, Kocaeli, Turkey
2Department of Stem Cell, Kocaeli University, Institue of Health Sciences, Kocaeli, Turkey
3Department of Medical Biology, Kocaeli University, Faculty of Medicine, Kocaeli, Turkey
4Department of Cardiovascular Surgery, University of Health Sciences, Derince Training and Research Hospital, Kocaeli, Turkey
5Department of Histology and Embryology, Kocaeli University, Faculty of Medicine, Kocaeli, Turkey
6Department of Pharmacology, Kocaeli University, Faculty of Medicine, Kocaeli, Turkey
7Department of Cardiovascular Surgery, Ministry of Health, Ankara City Hospital, Ankara, Turkey
DOI : 10.9739/tjvs.2022.1079 Objectives: In this study, we aimed to investigate the relationship between vascular system changes due to the increased inflammatory response and oxidative stress with advanced age and fetuin-A levels in aortic tissues of a naturally aged rat model.

Materials and methods: A total of 16 Wistar albino rats were equally divided into two groups as the young group and the elderly group. The thoracic aorta was excised. The effect of aging on the aorta, proliferation, oxidative stress, and inflammation markers were evaluated using the real-time polymerase chain reaction method. Histological examination was performed to confirm the findings related to aging, and serum sampling was performed to determine fetuin-A levels.

Results: Interleukin-6 levels were lower in the elderly group (1.007 vs. 0.099-fold decrease, respectively; p=0.000). There was a moderate, positive correlation between fetuin-A and interleukin-6 levels (r=0.56; p=0.03). There was no significant difference in the antioxidant capacity as assessed by superoxide dismutase-1, while the oxidative stress markers were significantly higher in elderly rats (inducible nitric oxide synthase: 1 vs. 812.3-fold increase, respectively; p=0.006; endothelial nitric oxide synthase: 0.98 vs. 3.65-fold increase, respectively; p=0.001). There was a moderate, negative correlation between fetuin-A and endothelial nitric oxide synthase (r=-0.56; p=0.024).

Conclusion: Vascular senescence causes cell damage through inflammatory responses. Fetuin-A may indicate early vascular damage. Keywords : Atherosclerosis, fetuin-A, inflammation, rat, senescence

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