THE EFFECT OF GADOLINIUM CHLORIDE ON RENAL INJURY IN THE MODEL OF EXPERIMENTAL AORTIC ISCHEMIA-REPERFUSION

Purpose: The aim of the present study was to examine the effect of gadolinium chloride (GdCl3) on aortic occlusion-reperfusion induced remote organ injury in kidney by assaying antioxidant enzymes in the kidney tissues.

Methods: Thirty-two rats were randomly allocated to four groups as follows: SHAM (sham laparotomy), SHAM + KCB (sham laparotomy + Kupffer cell blockage), AIR (aortic ischemia reperfusion) and AIR + KCB (aortic ischemia reperfusion + Kupffer cell blockage). GdCl3 was given 24 hours prior to experiment. An autraumatic microvascular clamp was placed across the infrarenal abdominal aorta (IAA) just after its origin from the aorta for 30 minutes. The microvascular clamp on the IAA was removed and reperfused for 60 minutes. Kidney malondialdeyde (MDA) level and myeloperoxidase (MPO), superoxide dismutase (SOD) and catalase (CAT) activities were assayed in the kidney tissues.

Results: MDA level and MPO, SOD and CAT activities in the AIR group were significantly higher than that in the other groups (p < 0.05). When compared to AIR group, KCB with GdCl3 significantly decreased MDA level and MPO, SOD and CAT activities in the AIR + KCB group (p < 0.05).

Conclusion: This experimental study showed that Kupffer cell blockage with GdCl3 attenuates ischemia-reperfusion injury in kidney induced by infrarenal aortic occlusion-reperfusion. (Turkish J Vasc Surg 2005;14(2):13-18).